Spermatogonial Stem Cells (SSCs) in Buffalo (Bubalus bubalis) Testis
نویسندگان
چکیده
منابع مشابه
Spermatogonial Stem Cells (SSCs) in Buffalo (Bubalus bubalis) Testis
BACKGROUND Water buffalo is an economically important livestock species and about half of its total world population exists in India. Development of stem cell technology in buffalo can find application in targeted genetic modification of this species. Testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo. OBJECTIVES A...
متن کاملIsolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were e...
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The aim of this study was to investigate the transcriptional profile and role of WNT3A signalling in maintaining buffalo embryonic stem (ES) cells in a pluripotent state and in the induction of their differentiation. ES cells were derived from embryos produced by in vitro fertilisation (iESC), parthenogenesis (pESC) and hand-made cloning (cESC). The expression of WNT3A, its receptors and interm...
متن کاملIn Vitro Culture of Buffalo (bubalus Bubalis) Spermatogonial Stem Cells: Effect of Serum, Sertoli Cell Coculture and Single Growth Factors
The objective of this study was to develop a short-term culture system for buffalo spermatogonial stem cells (SSCs) in various culture conditions. Spermatogonial stem cells as well as sertoli cells were isolated from prepubertal buffalo calf testes and enriched to culture for two weeks in a serum-free medium. The serum-free medium was supplemented with various concentrations of serum (FBS) or f...
متن کاملGeneration of induced pluripotent stem cells from buffalo (Bubalus bubalis) fetal fibroblasts with buffalo defined factors.
Ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem cells (iPSCs), which initiates a new strategy to obtain pluripotent stem cell lines. Attempts have been made to generate buffalo pluripotent stem cells by culturing primary germ cells or inner cell mass, but the efficiency is extremely low. Here, we report a successful method to repr...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0036020